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| 【资源】2007年 23卷 1期《 中华胸心血管外科杂志 》之三 |
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约瑟福
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 2008-06-25 20:59      2007年 23卷 1期《 中华胸心血管外科杂志 》 Chinese Journal of Thoracic and Cardiovascular Surgery起止页码：40-42国际标准刊号：ISSN 1001-4497国内统一刊号：CN 11-2434左肺反向循环动物模型的建立赵永红 苏肇伉 王顺民 张海波 陈恩上海交通大学附属新华医院上海儿童医学中心胸心外科,200127摘 要:目的探讨反向肺循环动物模型的建立方法及其可行性。方法选正常犬作为实验对象，利用牛颈静脉作为连接血管材料，建立左肺反向循环模型。于反向循环术前、术后2h、2周时测定血流动力参数和动脉血气，用超声流量仪测定左肺动脉流量。动物饲养到2周时行心血管造影检查，观察反向循环肺灌注情况，动物处死后取部分肺组织行病理学检查。结果正常犬行左肺反向循环术后2h、术后2周血流动力学无明显改变，并能维持正常的动脉血气，反向循环后左肺动脉流量无减少，心导管造影右侧肺动脉及其分支显影清晰，左侧牛颈静脉及肺静脉系统亦显影清晰，吻合口通畅。大体观察左、右肺无差别，组织病理学检查左、右肺亦无明显差异。结论左肺反向循环具有可行性，可维持良好的血流动力学、肺的正常结构和通气交换功能。[著者文摘] 关键词:肺循环 循环和呼吸生理学 模型 动物 分类号: R541.1[机标]文献标识码：文章编号：栏目信息：实验研究 相关文献:主题相关 全文快照 An aninml model of reversed pulmonary circulation in left lungZHAO Yong-hong, SU Zhao-kang, WANG Shtm-min, et al.Department of Pediatric Cardiothoracic Surgery, Xin Hua Hospital, Shanghai Children＇s Medical Center, Shanghai Jiaotong University, Shanghai 200127, ChinaAbstract:Objective To develop an experimental model of total inversion of the circulation of the left lung. With this model, we looked for the possibility of the total inversion of the lung blood flow, maintaining normal their functional and histological characteristics. Methods Normal mongrel dogs were subjected to reversal of blood flow to the left lung as an experimental model for total invetsion of lung circulation. Hemodynamic data and blood gas measurments were recorded before surgery, 2 hours after termination of the operation, and 2 weeks after the operation. The hemodynamics and flowmetry were performed. The animal was the sacrificed for histological evaluation, Results The gross appearance of reversed lung was normal, and there were no difference in blood flow and pulmonary arterial pressure between the normal and reversed lung. Blood gas exchange was normal, left pulmonary artery remained smooth without embolism. Conclusion With the total inversion of the circulation of the left lung, the function of gaseous exchange and the morphologic structure stayed unaffected. Our experiment shows that reversed pulmonary circulation can maintain lift in a canine model; it might be used as a tool for further studies of pulmonary vascular physiology.[著者文摘] Key words  ulmonary circulation Circulatory and respiratory physiology Models, animal
 左肺反向循环动物模型的建立.pdf (205.64k)
• 【共享】中国生物制品主要原辅材料质控标准（2000年版） |
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约瑟福
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2008-06-25 20:59 2007年 23卷 1期《 中华胸心血管外科杂志 》 Chinese Journal of Thoracic and Cardiovascular Surgery起止页码：43-45国际标准刊号：ISSN 1001-4497国内统一刊号：CN 11-2434VEGF121体外转染大鼠骨骼肌成肌细胞的实验研究徐磊 夏家红 张凯伦 谢艾妮华中科技大学同济医学院附属协和医院,武汉430022摘 要:目的研究血管内皮生长因子基因（VEGF121）在原代培养大鼠骨骼肌成肌细胞中的转染表达。方法通过脂质体介导将VEGF121基因转染到原代培养的大鼠骨骼肌成肌细胞中。经RT-PCR、Weaterrv Blot及免疫荧光检测转基因成肌细胞的基因表达情况。结果成肌细胞在基因转染后24h即出现VEGF121的表达，于48～72h达表达高峰，以后逐渐下调，维持表达2周左右。转染后的成肌细胞表达VEGF121蛋白质产物明显增加。结论经脂质体介导转染VEGF121基因的大鼠骨骼肌成肌细胞在体外有良好的因基表达，为VEGF基因修饰成肌细胞移植奠定基础。[著者文摘] 关键词:内皮,血管 内皮生长因子 RNA,转移 肌,骨骼 分类号: R554.305[机标]文献标识码：文章编号：栏目信息：实验研究 相关文献:主题相关 全文快照 Experimental study on the transfection and expression of vascular endothelial growth factor in primary cultured rat skeletal myoblastaXU Lei, XIA Jia-hong , ZHANG Kai-lun, et al .Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022,ChinaAbstract:Objective To investigate the transfection and expression of vascular endothelial growth factor in primary cultured rat skeletal myoblasts. Methods VEGF121 gene was transfected to primary cultured rat skeletal myoblasts by lipofectamine in vitro. Gene expression of transfected myoblasts was detected by RT-PCR, Western-Blot and immunofluorescent staining. Results Transleered myoblasts express VEGF121 gene in 24 hours, and the peak appears in 48 - 72 hours, and the transfected myoblasts continuously express gene during 2 weeks. Transfected myoblasts transfected with VEGF121 gene by lipofectamine can express gene products efficiently in vitro, which may lay basis to the transplantation of skeletal myoblasts transfected with VEGF gene. Conclusion.[著者文摘] Key words:Endothelium,vascular Endothelial growth factors RNA,transfer Muscle, skeletal VEGF121体外转染大鼠骨骼肌成肌细胞的实验研究.pdf (337.23k)
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约瑟福
医博园版主
发贴: 3844 积分: 442 得票:
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2008-06-25 20:59 2007年 23卷 1期《 中华胸心血管外科杂志 》 Chinese Journal of Thoracic and Cardiovascular Surgery起止页码：45-46国际标准刊号：ISSN 1001-4497国内统一刊号：CN 11-2434脉动流预适应增强组织工程心脏瓣叶上内皮细胞的黏附性王克学 张镜芳 简旭华广东省心血管病研究所心脏外科,广州510100摘 要:将受体的细胞种植于可降解材料或去细胞的异种或同种瓣膜上制成的组织工程心脏瓣膜无需抗凝，耐久性好，且有生长潜力，理论上是理想的心脏瓣膜替代物。但生理情况下，心脏瓣膜要受到高速、高压的血流冲击，简单的将内皮细胞种到瓣膜支架，经过血流冲击，细胞很快被冲掉。[第一段] 关键词:组织工程心脏瓣膜 内皮细胞 心脏瓣叶 黏附性 预适应 脉动流 可降解材料 同种瓣膜 分类号: R318.11 脉动流预适应增强组织工程心脏瓣叶上内皮细胞的黏附性.pdf (215.82k)
• 【翻译】这里怎么比较这些数据的 |
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约瑟福
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2008-06-25 21:00 2007年 23卷 1期《 中华胸心血管外科杂志 》 Chinese Journal of Thoracic and Cardiovascular Surgery起止页码：47-49国际标准刊号：ISSN 1001-4497国内统一刊号：CN 11-2434落新妇甙对心脏移植效应性T细胞磷脂酰肌醇信号通路的影响高思海 李平 陈涛 徐沁孜 宋定伟 刘力刚 潘铁成华中科技大学同济医学院附属同济医院胸心外科,武汉430030摘 要:目的探讨落新妇甙对心脏移植效应性T细胞磷脂酰肌醇-3-激酶／蛋白激酶B（PI-3K／PKB）信号传导通路的影响。方法分离BALB／C小鼠心肌细胞（2×10s／m1）和C57BL／6小鼠的脾细胞（1×10^6／ml），前者作刺激细胞，后者作反应细胞，进行混合细胞培养，建立小鼠心脏移植急性排斥反应体外模型。实验分4组：对照组，心肌细胞与脾细胞混合培养；3个实验组在对照组基础上，落新妇甙组加入落新妇甙（15μg/ml）；落新妇甙加P13K激动剂组加入落新妇甙（15μg/ml）和P13K激动剂IL-8（20μg/ml）；落新妇甙加P13K抑制剂组加入落新妇甙（15μg／ml）和P13K抑制剂LY294002（20μmol／L）。TUNEL法检测T淋巴细胞凋亡情况。Western blot法检测T细胞P13K和Bcl-2蛋白表达情况。RT-PCR法检测T细胞PKB mRNA表达情况。结果落新妇甙组T细胞凋亡指数明显高于对照组[（74．2±11．7）％对（34．2±9．6）％，P〈0．01]，P13K、PKB、Bcl-2表达显著低于对照组（P〈0．01）。落新妇甙加P13K抑制剂组T细胞凋亡指数显著高于落新妇甙组，P13K、PKB、Bcl-2显著低于落新妇甙组（P〈0．01）。落新妇甙加P13K激动剂组T细胞凋亡指数和Bcl-2表达与对照组的差异无统计学意义（P〉0．05）。结论落新妇甙诱导心脏移植效应性T细胞凋亡与其抑制PI-3K／PKB信号通路有关。[著者文摘] 关键词:心脏移植 移植物排斥 磷脂酰肌醇3-激酶 蛋白激酶类 落新妇甙 分类号: R735.7[机标]文献标识码：文章编号：栏目信息：实验研究 相关文献:主题相关 全文快照 Effect of astilbin on inhibition of phosphatidy linositol 3-kinase-protein kinase B（PI-3K-PKB）in alioreactive T cells of mouse heart transplantation model with acute rejectionGAO Si-hai , LI Ping, CHEN Tao , et al Department of Thoracic and Cardiac Surgery, Tangji Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430030, ChinaAbstract:Objective To investigate the effect of astilbin on inhibition of phosphatidy linositol 3-kinase-protein kinase B （PI-3K-PKB） in alloreacfive T cells of mouse heart transplantation model with acute rejection. Methods Cardlomyocytes of BALB/C mouse and spleen cells of C57BL/6 mouse were separated , the cardiomyocytes （2 × 10^5/ml）as stimulates and spleen cells （1× 10^6/ml） as responsers were mixed cultured. A model of mouse heart transplantation with acute rejection in vitro was established. There were four study groups. Control group, mixed culture of the cardiomyocytes and spleen cells; Astilbin group, mixed culture of the cardiomyocytes and spleen cells and astilbin（ 15 μg/ml ）. Astilbin ＋ IL-8 group, mixed culture of the cardiomyocytes and spleen cells and aatilbin（15 μg/ml ） and IL-8 （20μg/rnl）. Astilbin ＋ LY294002 group, mixed culture of the cardiomyocytes and spleen cells and aatilbin （15μg/ml） and LY294002 （20 μmol/L）. Apoptosis of T cedis were observed by TUNEL. The expression of P13K and Bcl- 2 in alloreactive T cells were measured by Western blot. The expression of PKB in alloreactive T cells was meaasured by RT-PCR. Results Apoptosis indexes in alloreactive T cells in Astilbin group were found significately higher than those of the control group [ （74.2 ＋±11.7） % vs. （ 34.2 ± 9.6 ） %, P 〈 0.01 ]. The expression of P13K, PKB and Bcl-2 in Astilbin group were significantly lower than control group （ P 〈 0.01 ）. The expression of P13K, PKB and Bcl-2 in Astilbin group were significantly higher than control group （ P 〈 0.01 ）. There were no significant difference between control group and Astilbin ＋ IL-8 group （ P 〉 0.05）. Conclusion The apoptosis of alloreactive T cells of heart transplantation induced by astilbin maybe partially related to its inhibition on expression expression of phosphatidy linositol 3-kinase-protein kinase B （PI-3K-PKB）.[著者文摘] Key words:Heart transplantation Graft rejection 1-Phosphatidylinositol-3-kinase Protein kinases Astilbin 落新妇甙对心脏移植效应性T细胞磷脂酰肌醇信号通路的影响.pdf (176.32k)
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约瑟福
医博园版主
发贴: 3844 积分: 442 得票:
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2008-06-25 21:00 2007年 23卷 1期《 中华胸心血管外科杂志 》 Chinese Journal of Thoracic and Cardiovascular Surgery起止页码：49-49国际标准刊号：ISSN 1001-4497国内统一刊号：CN 11-2434原发性肺纤维肉瘤1例陈厚坤 周新刚 宋巍哈尔滨医科大学附属第一医院心外科,黑龙江150001摘 要:病人女，39岁,左肩及胸背部痛伴咳嗽，深吸气时加重。x线胸片及CT检查显示左肺上叶后段有一直径约6．0锄圆形肿块，密度均匀，界限清晰，边缘规整，与后胸壁有部分粘连。CT值19HU。术前疑诊左肺间皮瘤。[第一段] 关键词:原发性肺纤维肉瘤 左肺上叶 圆形肿块 CT检查 x线胸片 胸背部 深吸气 CT值 分类号: R734.2 原发性肺纤维肉瘤1例.pdf (59.38k)
• 【公告】严肃考风考纪 维护公平公正 |
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约瑟福
医博园版主
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2008-06-25 21:01 2007年 23卷 1期《 中华胸心血管外科杂志 》 Chinese Journal of Thoracic and Cardiovascular Surgery起止页码：50-52国际标准刊号：ISSN 1001-4497国内统一刊号：CN 11-2434冠状动脉竞争血流对乳内动脉旁路血流及NO、ET含量影响的实验研究毕研文 付强 于建民 刘蔚 孙文宇 庞昕焱 乔丽山东大学齐鲁医院心外科,济南250012摘 要:目的研究冠状动脉旁路移植（CABG）术后，未完全闭塞冠状动脉的竞争血流对左乳内动脉（加雌）旁路血流量、方向及旁路血流中一氧化氮（NO）、内皮素（ET）含量的影响。方法15只猪行㈣术建立不同程度狭窄的冠状动脉左前降支（LAD）与LIMA旁路血管竞争血流动物模型，并检测LIMA旁路血流中NO、ET含量。结果LAD近端狭窄程度越轻，LIMA旁路平均血流量越小，且收缩期逆向血流越大，IAD近端30％狭窄、50％狭窄时LIMA旁路的血流量均明显低于90％狭窄时（P〈0．01）。IAD近端30％和50％狭窄时旁路血流NO含量均明显低于IAD近端90％狭窄时（P〈0．05）；LIMA旁路血流中ET含量均较移植前高（P〈0．05）。结论CABG术后，来自未完全闭塞冠状动脉的竞争血流导致加雌旁路血流量下降并产生双向血流，进而引起旁路血流中NO含量显著下降，可能是早期LIMA血管旁路闭塞的主要机制。[著者文摘] 关键词:冠状动脉分流术 乳房动脉 心肌血管重建术 移植物闭塞 血管 内皮缩血管肽类 一氧化氮 竞争血流 分类号: R654.2[机标]文献标识码：文章编号：栏目信息：实验研究 相关文献:主题相关 全文快照 Effects of competitive flow from differently stenotic coronary artery on the concentration of ET and NO in the blood in internal mammary artery graftBI Yan-wen, FU Qiang, YU Jian-min, et al. Department of Cardiovascular Surgery, Qilu Hospital of Shandong University Jinan 250012, ChinaAbstract:Objective To determine the effects of competitive flow from differently stenotic coronary artery on the flow, the ooncentration of nitric oxide （NO）, and endothelin （ET） in the blood of left internal mammary artery （LIMA） graft and factors that may be involved in early graft failure. Methods Fifteen Chinese swines underwent coronary artery bypass grafting using IMA to left anterior descending （LAD） artery. The procedure was performed using an ＂off pump＂ technique. Different stonosis was mad on proximid LAD through a flow occlusion. The mean flow of LIMA and LAD proximal and distal to the anastomosis and the concentration of El＂ and NO in the blood of LIMA graft were measured respectively. Results When the stenosis of the proximal LAD was 30% or .50%, the mean flow of LIMA was significant lower than that of 90% （ P 〈 0.01 ）. An oscillating flow （retrograde/antegrade） was noted in the LIMA graft. When the stenosis of the proximal LAD was 30% and 50%, the content of NO in blood of LIMA graft were significant lower than that of 90%. The content of ET in the blood of LIMA graft was significant higher than that before grafting（ P 〈 0.05）. Conclusion During competitive flow from nature LAD,low levels of flow and oscillating flow may be poorly tolerated by the LIMA etMothelium, contribute to the decrease in NO content, may contribute LIMA earlier graft failure.[著者文摘] Key words:Coronary artery bypass Mammary artery Myocardial vascularization Graft occlusion, vascular Endothelins Nitric oxide Competitive flow 冠状动脉竞争血流对乳内动脉旁路血流及NO、ET含量影响的实验研究.pdf (232.1k)
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约瑟福
医博园版主
发贴: 3844 积分: 442 得票:
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2008-06-25 21:01 2007年 23卷 1期《 中华胸心血管外科杂志 》 Chinese Journal of Thoracic and Cardiovascular Surgery起止页码：53-54国际标准刊号：ISSN 1001-4497国内统一刊号：CN 11-2434升主动脉-左心室流出道异常通道的诊断和治疗李京悻 程沛 罗毅 吴永涛 薛金熔首都医科大学附属北京安贞医院心外科,100029摘 要:升主动脉-左心室流出道异常通道（ALVT）是非常罕见的先天性心脏畸形，其发病率占先天性心脏病的0．11%．特征是主动脉瓣周存在异常通道，此通道并不进入肌部室间隔，通常开口在升主动脉和左心室流出道。[第一段] 关键词:左心室流出道 升主动脉 通道 先天性心脏畸形 治疗 诊断 主动脉瓣 发病率 分类号: R331.3 升主动脉-左心室流出道异常通道的诊断和治疗.pdf (356.0k)
• 【分享】痛性眼肌麻痹 |
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约瑟福
医博园版主
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2008-06-25 21:02 2007年 23卷 1期《 中华胸心血管外科杂志 》 Chinese Journal of Thoracic and Cardiovascular Surgery起止页码：54-54国际标准刊号：ISSN 1001-4497国内统一刊号：CN 11-2434胆固醇肺炎肺内孤立结节1例张义木 吴才 彭炯广东省农垦中心医院心胸外科,湛江524002摘 要:病人 女，41岁。体检发现左下肺阴影50余天，平素无发热，咳嗽、咳痰、胸痛。查体双肺未见异常。正位及左前余位胸部X线片示左下肺肿物，考虑良性肿瘤，错构瘤可能性大（图1.2）。[第一段] 关键词:肺内孤立结节 胆固醇肺炎 X线片示 良性肿瘤 肺阴影 肺肿物 错构瘤 分类号: R734.2 胆固醇肺炎肺内孤立结节1例.pdf (114.43k)
• 【转载】SCI收录的《中国神经再生研究（英文版）》(Neural Regeneration Research NRR)杂志的学术理念 |
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