【有奖翻译】重组基因表达原理和方法手册

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【有奖翻译】重组基因表达原理和方法手册

番茄老人

医博园版主

发贴: 1919
积分: 311

得票: 42

状态: 隐身

2008-01-22 20:03

现征集翻译英文版的重组基因表达原理和方法手册，本书总共33部，里面有很详细介绍了基因表达重组的原理和材料方法，是一本很有价值的实验手册，请大家踊跃参与，每一部分都有20-30页不等，根据内容的长短将按每个章节10-15个积分给以奖励，每个人可以认领一个或多个章节。凡来认领的会员均可得1个积分，第一个认领给以2个积分鼓励，但是认领后没有交上来，我们将收回这个积分。我们希望和争取能在今年下半年打造出分子生物版的第一本实验手册。认领的会员，请跟帖并注明认领的章节，完成后也来此帖说明，为了防止我们辛勤的劳动果实被下载盗取，建议不要把翻译后的文章直接链接上来，发到E-mail:yby123@gmail.com，我们将给以校正，如果能够出册子的话，到时向管理员申请，争取给每位作者酬劳。

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1.Host Cell Compatibility in Protein Expression
2.Production of Recombinant Proteins
3.Folding-Promoting Agents in Recombinant Protein Production
4.Back to Basics：pBR322 and Protein Expression Systems in E. coli
5.α-Complementation-Enabled T7 Expression Vectors and Their Use for the Expression of Recombinant Polypeptides for Protein Transduction Experiments
6.Expression of Recombinant Alkaline Phosphatase Conjugates in Escherichia coli
7.Overexpression of Chromosomal Genes in Escherichia coli
8.Chromosomal Expression of Foreign and Native Genes From Regulatable Promoters in Escherichia coli
9.Plasmid Vectors for Marker-Free Chromosomal Insertion of Genetic Material in Escherichia coli
10.Copy-Control pBAC/oriV Vectors for Genomic Cloning
11.Copy-Control Tightly Regulated Expression Vectors Based on pBAC/oriV
12.Cell-Free Protein Synthesis With Prokaryotic Combined Transcription-Translation
13.Genetic Tools for the Manipulation of Moderately Halophilic Bacteria of the Family Halomonadaceae
14.Gene Transfer and Expression of Recombinant Proteins in Moderately Halophilic Bacteria
15.Recombinant Protein Production in Antarctic Gram-Negative Bacteria
16.Recombinant Protein Production in Yeasts
17.Controlled Expression of Homologous Genes by Genomic Promoter Replacement in the Yeast Saccharomyces cerevisiae
18.High-Throughput Expression in Microplate Format in Saccharomyces cerevisiae
19.High-Throughput Expression in Microplate Format in Pichia pastoris
20.Multiple Gene Expression by Chromosomal Integration and CRE-loxP-Mediated Marker Recycling in Saccharomyces cerevisiae
21.Three Decades of Fungal Transformation：Key Concepts and Applications
22.Three Decades of Fungal Transformation：Novel Technologies
23.Gene Transfer and Expression in Plants
24.Production of Recombinant Proteins by Hairy Roots Cultured in Plastic Sleeve Bioreactors
25.Engineering the Chloroplast Genome for Hyperexpression of Human Therapeutic Proteins and Vaccine Antigens
26.New Selection Marker for Plant Transformation
27.Enhancer Detection and Gene Trapping as Tools for Functional Genomics in Plants
28.Gene Transfer and Expression in Mammalian Cell Lines and Transgenic Animals
29.Sustained Heterologous Transgene Expression in Mammalian and Avian Cell Lines
30.Inducible Gene Expression in Mammalian Cells and Mice
31.Flp-Mediated Integration of Expression Cassettes into FRT-Tagged Chromosomal Loci in Mammalian Cells
32.Generation of High-Recombinant-Protein-Producing Chinese Hamster Ovary (CHO) Cells
33.Preparation of Recombinant Proteins in Milk