【资源】CURRENT PROTOCOLS IN CELL BIOLOGY

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【资源】CURRENT PROTOCOLS IN CELL BIOLOGY

番茄老人

医博园版主

发贴: 1919
积分: 311

得票: 42

状态: 隐身

2008-02-10 22:09

目录：

CURRENT PROTOCOLS IN CELL BIOLOGY
FRONT MATTER
PUBLICATION INFORMATION
FOREWORD
PREFACE
CONTRIBUTORS

CHAPTER 1. CELL CULTURE
CHAPTER 1. INTRODUCTION
UNIT 1.1 Basic Techniques for Mammalian Cell Tissue Culture
UNIT 1.2 Media for Culture of Mammalian Cells
UNIT 1.3 Aseptic Technique for Cell Culture
UNIT 1.4 Sterilization and Filtration
UNIT 1.5 Assessing and Controlling Microbial Contamination in Cell Cultures
UNIT 1.6 Media and Culture of Yeast

CHAPTER 2. PREPARATION AND ISOLATION OF CELLS
CHAPTER 2. INTRODUCTION
UNIT 2.1 Establishment of Fibroblast Cultures
UNIT 2.2 Preparation and Culture of Human Lymphocytes
UNIT 2.3 Preparation of Endothelial Cells
UNIT 2.4 Generation of Continuously Growing B Cell Lines by Epstein-Barr Virus Transformation
UNIT 2.5 Laser Capture Microdissection

CHAPTER 3. SUBCELLULAR FRACTIONATION AND ISOLATION OF ORGANELLES
CHAPTER 3. INTRODUCTION
UNIT 3.1 Overview of Cell Fractionation
UNIT 3.2 Isolation of Rat Hepatocyte Plasma Membrane Sheets and Plasma Membrane Domains
UNIT 3.3 Isolation of Mitochondria from Tissues and Cells by Differential Centrifugation
UNIT 3.4 Purification of a Crude Mitochondrial Fraction by Density-Gradient Centrifugation
UNIT 3.5 Isolation of Peroxisomes from Tissues and Cells by Differential and Density Gradient Centrifugation
UNIT 3.6 Isolation of Lysosomes from Tissues and Cells by Differential and Density Gradient Centrifugation
UNIT 3.7 Overview of Subcellular Fractionation Procedures for the Yeast Saccharomyces cerevisiae
UNIT 3.8 Isolation of Subcellular Fractions from the Yeast Saccharomyces cerevisiae
UNIT 3.9 Isolation of Golgi Membranes from Tissues and Cells by Differential and Density Gradient Centrifugation
UNIT 3.10 Isolation of Nuclei and Nuclear Membranes From Animal Tissues

CHAPTER 4. MICROSCOPY
CHAPTER 4. INTRODUCTION
UNIT 4.1 Proper Alignment and Adjustment of the Light Microscope
UNIT 4.2 Fluorescence Microscopy
UNIT 4.3 Immunofluorescence Staining
UNIT 4.4 Fluorescent Staining of Subcellular Organelles: ER, Golgi Complex, and Mitochondria
UNIT 4.5 Basic Confocal Microscopy
UNIT 4.6 Immunoperoxidase Methods for Localization of Antigens in Cultured Cells and Tissues
UNIT 4.7 Cryo-Immunogold Electron Microscopy
UNIT 4.8 Correlative Video Light/Electron Microscopy
UNIT 4.9 Polarization Microscopy
UNIT 4.10 Fluorescent Speckle Microscopy (FSM) of Microtubules and Actin in Living Cells

ORGANELLE ATLAS
APPENDIX TO CHAPTER 4
FIGURE(S)

CHAPTER 5. CHARACTERIZATION OF CELLULAR PROTEINS
CHAPTER 5. INTRODUCTION
UNIT 5.1 Overview of the Physical State of Proteins Within Cells
UNIT 5.2 Determining the Topology of an Integral Membrane Protein
UNIT 5.3 Determination of Molecular Size by Zonal Sedimentation Analysis on Sucrose Density Gradients
UNIT 5.4 Analysis of the Association of Proteins with Membranes
UNIT 5.5 Determination of Molecular Size by Size-Exclusion Chromatography (Gel Filtration)

CHAPTER 6. ELECTROPHORESIS AND IMMUNOBLOTTING
CHAPTER 6. INTRODUCTION
UNIT 6.1 One-Dimensional SDS Gel Electrophoresis of Proteins
UNIT 6.2 Immunoblotting and Immunodetection
UNIT 6.3 Detection and Quantitation of Radiolabeled Proteins in Gels and Blots
UNIT 6.4 Two-Dimensional Gel Electrophoresis
UNIT 6.5 One-Dimensional Electrophoresis Using Nondenaturing Conditions
UNIT 6.6 Staining Proteins in Gels

CHAPTER 7. PROTEIN LABELING AND IMMUNOPRECIPITATION
CHAPTER 7. INTRODUCTION
UNIT 7.1 Metabolic Labeling with Amino Acids
UNIT 7.2 Immunoprecipitation
UNIT 7.3 Metabolic Labeling with Sulfate
UNIT 7.4 Metabolic Labeling with Fatty Acids
UNIT 7.5 Metabolic Labeling of Prenyl and Carboxyl-Methyl Groups
UNIT 7.6 Metabolic Labeling and Immunoprecipitation of Yeast Proteins
UNIT 7.7 Metabolic Labeling and Immunoprecipitation of Drosophila Proteins
UNIT 7.8 Metabolic Labeling of Glycoproteins with Radioactive Sugars

CHAPTER 8. CELL CYCLE ANALYSIS
CHAPTER 8. INTRODUCTION
UNIT 8.1 Overview of the Cell Cycle
UNIT 8.2 Assays for CDK Activity and DNA Replication in the Cell Cycle
UNIT 8.3 Methods for Synchronizing Cells at Specific Stages of the Cell Cycle
UNIT 8.4 Determining Cell Cycle Stages by Flow Cytometry
UNIT 8.5 Centrifugal Elutriation to Obtain Synchronous Populations of Cellsab

CHAPTER 9. CELL ADHESION
CHAPTER 9. INTRODUCTION
UNIT 9.1 Cell-Substrate Adhesion Assays
UNIT 9.2 Quantitative Measurement of Cell Adhesion Using Centrifugal Force
UNIT 9.3 Cadherin-Dependent Cell-Cell Adhesion
UNIT 9.4 Analyzing Integrin-Dependent Adhesion
UNIT 9.5 Analysis of Cell-Cell Contact Mediated by Ig Superfamily Cell Adhesion Molecules

CHAPTER 10. EXTRACELLULAR MATRIX
CHAPTER 10. INTRODUCTION
UNIT 10.1 Overview of Extracellular Matrix
UNIT 10.2 Preparation of Basement Membrane Components from EHS Tumors
UNIT 10.3 Preparation of Gelled Substrates
UNIT 10.4 Preparation of Extracellular Matrices Produced by Cultured Corneal Endothelial and PF-HR9 Endodermal Cells
UNIT 10.5 Purification of Fibronectin
UNIT 10.6 Purification of Vitronectin
UNIT 10.7 Proteoglycan Isolation and Analysis
UNIT 10.8 Matrix Metalloproteinases

CHAPTER 11. IN VITRO RECONSTITUTION
CHAPTER 11. INTRODUCTION
UNIT 11.1 Overview of Eukaryotic In Vitro Translation and Expression Systems
UNIT 11.2 In Vitro Translation
UNIT 11.3 In Vitro Analysis of Endoplasmic-Reticulum-to-Golgi Transport in Mammalian Cells
UNIT 11.4 Cotranslational Translocation of Proteins into Canine Rough Microsomes
UNIT 11.5 In Vitro Analysis of SV40 DNA Replication
UNIT 11.6 In Vitro Transcription
UNIT 11.7 Nuclear Import in Digitonin-Permeabilized Cells
UNIT 11.8 In Vitro Translation Using HeLa Extract
UNIT 11.9 Analysis of Eukaryotic Translation in Purified and Semipurified Systems
UNIT 11.10 Preparation and Use of Interphase Xenopus Egg Extracts
UNIT 11.11 Analysis of the Cell Cycle Using Xenopus Egg Extracts
UNIT 11.12 Analysis of Apoptosis Using Xenopus Egg Extracts
UNIT 11.13 Mitotic Spindle Assembly In Vitro
UNIT 11.14 Analysis of RNA Export Using Xenopus Oocytes

CHAPTER 12. CELL MOTILITY
CHAPTER 12. INTRODUCTION
UNIT 12.1 Chemotaxis Assays for Eukaryotic Cells
UNIT 12.2 Invasion Assays
UNIT 12.3 Cell Traction
UNIT 12.4 Cell Wound Assays
UNIT 12.5 Dictyostelium Cell Dynamics

CHAPTER 13. ORGANELLE MOTILITY
CHAPTER 13. INTRODUCTION
UNIT 13.1 Microtubule/Organelle Motility Assays
UNIT 13.2 In Vitro Motility Assay to Study Translocation of Actin by Myosin
UNIT 13.3 Organelle Motility in Plant Cells: Imaging Golgi and ER Dynamics with GFP
UNIT 13.4 Movement of Nuclei

CHAPTER 14. SIGNAL TRANSDUCTION: PROTEIN PHOSPHORYLATION
CHAPTER 14. INTRODUCTION
UNIT 14.1 Overview of Protein Phosphorylation
UNIT 14.2 Immunological Detection of Phosphorylation
UNIT 14.3 Detection of MAP Kinase Signaling
UNIT 14.4 Labeling Cultured Cells with 32Pi and Preparing Cell Lysates for Immunoprecipitation
UNIT 14.5 Phosphoamino Acid Analysis

CHAPTER 15. PROTEIN TRAFFICKING
CHAPTER 15. INTRODUCTION
UNIT 15.1 Overview of Protein Trafficking in the Secretory and Endocytic Pathways
UNIT 15.2 Use Of Glycosidases To Study Protein Trafficking
UNIT 15.3 Endocytosis: Biochemical Analyses
UNIT 15.4 Determining Protein Transport to the Plasma Membrane
UNIT 15.5 Analysis of Membrane Traffic in Polarized Epithelial Cells
CHAPTER 16. ANTIBODIES AS CELL BIOLOGICAL TOOLS

CHAPTER 16. ANTIBODIES AS CELL BIOLOGICAL TOOLS
CHAPTER 16. INTRODUCTION
UNIT 16.1 Production of Monoclonal Antibodies
UNIT 16.2 Production of Polyclonal Antisera
UNIT 16.3 Purification of Immunoglobulin G
UNIT 16.4 Fragmentation of Immunoglobulin G
UNIT 16.5 Antibody Conjugates for Cell Biology
UNIT 16.6 Production of Antibodies That Recognize Specific Tyrosine-Phosphorylated Peptides

CHAPTER 17. MACROMOLECULAR INTERACTIONS IN CELLS
CHAPTER 17. INTRODUCTION
UNIT 17.1 Imaging Protein-Protein Interactions by Fluorescence Resonance Energy Transfer (FRET) Microscopy
UNIT 17.2 Identification of Protein Interactions by Far Western Analysis
UNIT 17.3 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins

CHAPTER 18. CELLULAR AGING AND DEATH
CHAPTER 18. INTRODUCTION
UNIT 18.1 Current Concepts in Cell Death
UNIT 18.2 Analysis of Caspase Activation During Apoptosis
UNIT 18.3 Assessment of Apoptosis and Necrosis by DNA Fragmentation and Morphological Criteria
UNIT 18.4 Quantitative Fluorescence In Situ Hybridization (Q-FISH)

CHAPTER 19. WHOLE ORGANISM AND TISSUE ANALYSIS
CHAPTER 19. INTRODUCTION
UNIT 19.1 Overview of Metastasis Assays
UNIT 19.2 Tail Vein Assay of Cancer Metastasis

APPENDIX 1 USEFUL INFORMATION AND DATA
APPENDIX 1A Useful Measurements and Data
APPENDIX 1B Compendium of Drugs Commonly Used in Cell Biology Research
APPENDIX 1C Identification of Motifs in Protein Sequences
APPENDIX 1D Safe Use of Radioisotopes
APPENDIX 1E Absorption and Emission Maxima for Common Fluorophores
APPENDIX 1F Importing Biological Materials
APPENDIX 1G Centrifuges and Rotors
APPENDIX 1H Internet Basics for Biologists

APPENDIX 2 LABORATORY STOCK SOLUTIONS AND EQUIPMENT
APPENDIX 2A Common Stock Solutions, Buffers, and Media
APPENDIX 2B Medium Formulations
APPENDIX 2C Standard Laboratory Equipment

APPENDIX 3 COMMONLY USED TECHNIQUES
APPENDIX 3A Molecular Biology Techniques
APPENDIX 3B Spectrophotometric and Colorimetric Determination of Protein Concentration
APPENDIX 3C Dialysis and Concentration of Protein Solutions
APPENDIX 3D Quantification of DNA and RNA with Absorption and Fluorescence Spectroscopy
APPENDIX 3E Silanizing Glassware
APPENDIX 3F Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization
APPENDIX 3G Micro RT-PCR

番茄老人 edited on 2008-06-11 18:46

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番茄老人

医博园版主

发贴: 1919
积分: 311

得票: 42

状态: 隐身

2008-02-10 22:17

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