【资源】The Protein Protocols Handbook-2nd Edition

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【资源】The Protein Protocols Handbook-2nd Edition

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80 Peptide Mapping by Sodium Dodecyl Sulfate-Polyacrylamide
Gel Electrophoresis
Ralph C. Judd .......................................................................................553
81 Peptide Mapping by High-Performance Liquid Chromatography
Ralph C. Judd .......................................................................................559
82 Production of Protein Hydrolysates Using Enzymes
John M. Walker and Patricia J. Sweeney .........................................563
83 Amino Acid Analysis by Precolumn Derivatization with 1-Fluoro-2,4-
Dinitrophenyl-5-L-Alanine Amide (Marfey's Reagent)
Sunil Kochhar, Barbara Mouratou, and Philipp Christen .............. 567
84 Molecular Weight Estimation for Native Proteins Using
High-Performance Size-Exclusion Chromatography
G. Brent Irvine ......................................................................................573
85 Detection of Disulfide-Linked Peptides by HPLC
Alastair Aitken and Michèle P. Learmonth ...................................... 581
86 Detection of Disulfide-Linked Peptides by Mass Spectrometry
Alastair Aitken and Michèle P. Learmonth ...................................... 585
87 Diagonal Electrophoresis for Detecting Disulfide Bridges
Alastair Aitken and Michèle P. Learmonth ...................................... 589
88 Estimation of Disulfide Bonds Using Ellman's Reagent
Alastair Aitken and Michèle P. Learmonth ...................................... 595
89 Quantitation of Cysteine Residues and Disulfide Bonds
by Electrophoresis
Alastair Aitken and Michèle P. Learmonth ...................................... 597
90 Analyzing Protein Phosphorylation
John Colyer ..........................................................................................603
91 Mass Spectrometric Analysis of Protein Phosphorylation
Débora BoneNfant, Thierry Mini, and Paul Jenö............................. 609
92 Identification of Proteins Modified by Protein
(D-Aspartyl/L-Isoaspartyl) Carboxyl Methyltransferase
Darin J. Weber and Philip N. McFadden...........................................623
93 Analysis of Protein Palmitoylation
Morag A. Grassie and Graeme Milligan ............................................633
94 Incorporation of Radiolabeled Prenyl Alcohols and Their Analogs
into Mammalian Cell Proteins: A Useful Tool for Studying
Protein Prenylation
Alberto Corsini, Christopher C. Farnsworth, Paul McGeady, Michael
H. Gelb, and John A. Glomset ........................................................641
95 The Metabolic Labeling and Analysis of Isoprenylated Proteins
Douglas A. Andres, Dean C. Crick, Brian S. Finlin,
and Charles J. Waechter .............................................................. 657
96 2-D Phosphopeptide Mapping
Hikaru Nagahara, Robert R. Latek, Sergi A. Ezhevsky,
and Steven F. Dowdy, ......................................................................673
97 Detection and Characterization of Protein Mutations
by Mass Spectrometry
Yoshinao Wada ....................................................................................681
98 Peptide Sequencing by Nanoelectrospray Tandem Mass Spectrometry
Ole Nørregaard Jensen and Matthias Wilm .....................................693
99 Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
for Protein Identification Using Peptide and Fragmention Masses
Paul L. Courchesne and Scott D. Patterson ................................... 711
100 Protein Ladder Sequencing
Rong Wang and Brian T. Chait .........................................................733
101 Sequence Analysis with WinGene/WinPep
Lars Hennig .........................................................................................741
102 Isolation of Proteins Cross-linked to DNA by Cisplatin
Virginia A. Spencer and James R. Davie.........................................747
103 Isolation of Proteins Cross-linked to DNA by Formaldehyde
Virginia A. Spencer and James R. Davie.........................................753
PART VI : GLYCOPROTEINS
104 Detection of Glycoproteins in Gels and Blots
Nicolle H. Packer, Malcolm S. Ball, Peter L. Devine,
and Wayne F. Patton .......................................................................761
105 Staining of Glycoproteins/Proteoglycans in SDS-Gels
Holger J. Møller and Jørgen H. Poulsen .........................................773
106 Identification of Glycoproteins on Nitrocellulose Membranes
Using Lectin Blotting
Patricia Gravel.....................................................................................779
107 A Lectin-Binding Assay for the Rapid Characterization
of the Glycosylation of Purified Glycoproteins
Mohammad T. Goodarzi, Angeliki Fotinopoulou,
and Graham A. Turner.....................................................................795
108 Chemical Methods of Analysis of Glycoproteins
Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith ...... 803
109 Monosaccharide Analysis by HPAEC
Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith ...... 805
110 Monosaccharide Analysis by Gas Chromatography (GC)
Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith ...... 809
111 Determination of Monosaccharide Linkage and Substitution
Patterns by GC-MS Methylation Analysis
Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith ...... 811
112 Sialic Acid Analysis by HPAEC-PAD
Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith ..... 815
113 Chemical Release of O-Linked Oligosaccharide Chains
Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith ..... 817
114 O-Linked Oligosaccharide Profiling by HPLC
Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith ..... 819
115 O-Linked Oligosaccharide Profiling by HPAEC-PAD
Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith ..... 821
116 Release of N-Linked Oligosaccharide Chains by Hydrazinolysis
Tsuguo Mizuochi and Elizabeth F. Hounsell ................................. 823
117 Enzymatic Release of O- and N-Linked Oligosaccharide Chains
Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith ...... 827
118 N-Linked Oligosaccharide Profiling by HPLC on Porous
Graphitized Carbon (PGC)
Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith ..... 829
119 N-Linked Oligosaccharide Profiling by HPAEC-PAD
Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith ..... 831
120 HPAEC-PAD Analysis of Monosaccharides Released by
Exoglycosidase Digestion Using the CarboPac MA1 Column
Michael Weitzhandler Jeffrey Rohrer, James R. Thayer,
and Nebojsa Avdalovic ...................................................................833
121 Microassay Analyses of Protein Glycosylation
Nicky K.C. Wong, Nnennaya Kanu, Natasha Thandrayen,
Geert Jan Rademaker, Christopher I. Baldwin,
David V. Renouf, and Elizabeth F. Hounsell.................................. 841
122 Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled
Carbohydrates from Glycoproteins
Brian K. Brandley, John C. Klock, and Christopher M. Starr ........ 851
123 HPLC Analysis of Fluorescently Labeled Glycans
Tony Merry..........................................................................................865
124 Glycoprofiling Purified Glycoproteins Using Surface Plasmon Resonance
Angeliki Fotinopoulou and Graham A. Turner ..............................885
125 Sequencing Heparan Sulfate Saccharides
Jeremy E. Turnbull ............................................................................893
126 Analysis of Glycoprotein Heterogeneity by Capillary Electrophoresis
and Mass Spectrometry
Andrew D. Hooker and David C. James ........................................905
127 Affinity Chromatography of Oligosaccharides and Glycopeptides
with Immobilized Lectins
Kazuo Yamamoto, Tsutomu Tsuji, and Toshiaki Osawa ............917
PART VII : ANTIBODY TECHNIQUES
128 Antibody Production
Robert Burns ......................................................................................935
129 Production of Antibodies Using Proteins in Gel Bands
Sally Ann Amero, Tharappel C. James, and Sarah C.R. Elgin ....... 941
130 Raising Highly Specific Polyclonal Antibodies Using Biocompatible
Support-Bound Antigens
Monique Diano and André Le Bivic ................................................945
131 Production of Antisera Using Peptide Conjugates
Thomas E. Adrian ..............................................................................953
132 The Chloramine T Method for Radiolabeling Protein
Graham S. Bailey ...............................................................................963
133 The Lactoperoxidase Method for Radiolabeling Protein
Graham S. Bailey ...............................................................................967
134 The Bolton and Hunter Method for Radiolabeling Protein
Graham S. Bailey ...............................................................................969
135 Preparation of 125I Labeled Peptides and Proteins with High
Specific Activity Using IODO-GEN
J. Michael Conlon ..............................................................................971
136 Purification and Assessment of Quality of Radioiodinated Protein
Graham S. Bailey ...............................................................................979
137 Purification of IgG by Precipitation with Sodium Sulfate
or Ammonium Sulfate
Mark Page and Robin Thorpe ..........................................................983
138 Purification of IgG Using Caprylic Acid
Mark Page and Robin Thorpe ..........................................................985
139 Purification of IgG Using DEAE-Sepharose Chromatography
Mark Page and Robin Thorpe ..........................................................987
140 Purification of IgG Using Ion-Exchange HPLC
Carl Dolman, Mark Page, and Robin Thorpe ................................. 989
141 Purification of IgG by Precipitation with Polyethylene Glycol (PEG)
Mark Page and Robin Thorpe ..........................................................991
142 Purification of IgG Using Protein A or Protein G
Mark Page and Robin Thorpe ..........................................................993
143 Analysis and Purification of IgG Using Size-Exclusion High
Performance Liquid Chromatography (SE-HPLC)
Carl Dolman and Robin Thorpe .......................................................995
144 Purification of IgG Using Affinity Chromatography
on Antigen-Ligand Columns
Mark Page and Robin Thorpe ..........................................................999
145 Purification of IgG Using Thiophilic Chromatography
Mark Page and Robin Thorpe .......................................................... 1003
146 Analysis of IgG Fractions by Electrophoresis
Mark Page and Robin Thorpe .......................................................... 1005
147 Purification of Immunoglobulin Y (IgY) from Chicken Eggs
Christopher R. Bird and Robin Thorpe .......................................... 1009
148 Affinity Purification of Immunoglobulins Using Protein A
Mimetic (PAM)
Giorgio Fassina, Giovanna Palombo, Antonio Verdoliva,
and Menotti Ruvo .............................................................................. 1013
149 Detection of Serological Cross-Reactions by Western Cross-Blotting
Peter Hammerl, Arnulf Hartl, Johannes Freund,
and Josef Thalhamer ...................................................................... 1025
150 Bacterial Expression, Purification, and Characterization
of Single-Chain Antibodies
Sergey M. Kipriyanov ........................................................................ 1035
151 Enzymatic Digestion of Monoclonal Antibodies
Sarah M. Andrew ..............................................................................1047
152 How to Make Bispecific Antibodies
Ruth R. French ................................................................................... 1053
153 Phage Display: Biopanning on Purified Proteins and Proteins
Expressed in Whole Cell Membranes
George K. Ehrlich, Wolfgang Berthold, and Pascal Bailon .......... 1059
154 Screening of Phage Displayed Antibody Libraries
Heinz Dörsam, Michael Braunagel, Christian Kleist,
Daniel Moynet, and Martin Welschof ........................................... 1073
155 Antigen Measurements Using ELISA
William Jordan .................................................................................1083
156 Enhanced Chemiluminescence Immunoassay
Richard A.W. Stott ...........................................................................1089
157 Immunoprecipitation
Kari Johansen and Lennart Svensson .........................................1097
PART VIII: MONOCLONAL ANTIBODIES
158 Immunogen Preparation and Immunization Procedures
for Rats and Mice
Mark Page and Robin Thorpe ........................................................1109
159 Hybridoma Production
Mark Page and Robin Thorpe ........................................................1111
160 Screening Hybridoma Culture Supernatants Using Solid-Phase
Radiobinding Assay
Mark Page and Robin Thorpe ....................................................... 1115
161 Screening Hybridoma Culture Supernatants Using ELISA
Mark Page and Robin Thorpe ........................................................1117
162 Growth and Purification of Murine Monoclonal Antibodies
Mark Page and Robin Thorpe ........................................................1119
163 Affinity Purification Techniques for Monoclonal Antibodies
Alexander Schwarz..........................................................................1121
164 A Rapid Method for Generating Large Numbers of High-Affinity
Monoclonal Antibodies from a Single Mouse
Nguyen Thi Man and Glenn E. Morris ...........................................1129
Index ...........................................................................................................1139

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发贴: 1919
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状态: 隐身

2008-02-13 12:47

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